attp attb recombination – attb sequence
A powerful method combining homologous recombination and
· Attachment sites attP and attB are substrates for Integrase Int mediated site-specific recombination to generate attachment sites attL and attR as products attL and attR are used as substrates for integrase-mediated excision but only in the present of the recombination directionality factor RDF Bxb1 gp47
The streptomyces genome contains multiple pseudo-attB
Thus secondary or pseudo-attB sites were identified by Southern blotting and after rescue of plasmids containing DNA flanking the insertion sites from the chromosome The sequences of the integration sites had similarity to those of attB Analysis of the insertions of pSET152 into both attB+ and DeltaattB strains indicated that this plasmid can integrate at several loci via independent recombination events within a …
· Bischof et al showed that a 54-bp attP supported recombination with a larger attB Results from using our M40 construct suggest that a 40-bp attB can efficiently recombine with a larger attP Therefore a 40-bp attB and a 54-bp attP might be sufficient for efficient integration in Drosophila Additional Utilities of SIRT
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Intrachromosomal recombination between attP regions as a
Only attP-attB recombination was observed when all three sites were present In the absence of the attB site a low frequency of attP-attL recombination was observed To demonstrate the functionality of the system the xylose utilization genes xylABR and xylT from L, lactis strain KF147 were integrated into the chromosome of L, lactis strain MG1363 in two steps, Skip to main content, ASM
· λ phage attB × attP recombination using the suicide conjugative transfer assay To confirm that these results truly reflect a single strand preference we investigated the related phage λ recombination system that is known to require two strands Here orientation should have no effect on recombination, We used the λ phage integration, since its mechanism is known in detail reviewed by
Repetitive Marker-Free Site-Specific Integration as a
This resulted in the formation of chimeric plasmids by site-specific recombination between the cloned attB and attP sequences The results indicate that the integration vector system based on the site-specific recombination mechanism of phage φFC1 can be used for genetic engineering in …
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Site-Specific Recombination of Temperate Myxococcus
Att site sequences
Streptomyces phage φC31 integrase induces efficient site-specific recombination capable of integrating exogenous genes at pseudo attP sites in human mouse rat rabbit sheep Drosophila and bovine genomes, However, the φC31-mediated recombination between attB and the corresponding pseudo attP sites has not been investigated in Capra hircus, Here, we identified eight pseudo attP sites located in the intron or …
A phage integrase directs efficient site-specific
· Below are all of the att sequences attB, P, L, and R used in the original three-part multisite Gateway system, These sequences come from the Invitrogen documentation, and we have seen a few single-base differences from the actual sequences of our clones it’s unclear whether these are documentation mistakes or mutations that have accumulated over time, Some of these differences are indicated by …
Introduction
attp attb recombination
· Phage integrases carry out recombination between attachment sites on the phage and bacterial genomes known as attP and attB respectively For phage φC31 the attP site previously was localized to an 84-bp fragment whereas the attB site was present on a 05-kb fragment 7–9, The minimal sizes of the attP and attB sites are determined in this paper,
Construction of an Integration-Proficient Vector Based on
Attb Recombination Site
Integration of bacteriophage λ into the Escherichia coli genome occurs by recombination between the phage attachment attP and the bacterial attachment attB regions
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PhiC31 integrase induces efficient site-specific
Recombination events between the donor and genome can occur between one or two attP–attB pairs leading to total of eight possible recombination outcomes Fig 3 All integration events between one attP–attB pair Fig 3 5–8, will eventually lead to another recombination event because of the presence of integrase and a second pair of intact attP and attB sites,
Integron cassette insertion: a recombination process
Attb Recombination Site, supplied by PrimerDesign Inc, used in various techniques, Bioz Stars score: 80/100, based on 0 PubMed citations, ZERO BIAS – scores, article reviews, protocol conditions and more
Int promotes attP × attB recombination more efficiently than attR × attB attL × attB or attB × attB recombination Prophage integration depends on a recombination reaction that is catalyzed by integrase and that proceeds in the highly favored forward direction attP + attB → attR + attL
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