cx3cr1 cre ert2 – cx3cr1 microglia

Cx3cr1 CreERT2 mice express Cre recombinase in a tamoxifen-inducible manner and have been widely used to delete target genes in microglia since microglia are long-lived cells and outlive peripheral macrophages which continuously turn over and lose their gene modification over time ATG7 is an E1-like enzyme that plays an essential role in two ubiquitin-like reactions, ATG12-ATG5 conjugation

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Protocol for genotyping LE-TgCX3CR1-Cre-ERT23Ottc

 · For induction of Cre recombinase 4–6-week-old Cx3cr1-Cre ERT2 mice were injected s,c with 2 mg tamoxifen Sigma USA solved in 100 μl corn oil Wako, Japan once a day for 2 days, All mice

Fate Mapping Reveals Origins and Dynamics of Monocytes and

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Cx3cr1 CreER knock-in/knock-out mice express a Cre-ERT2 fusion protein and EYFP in microglia in the brain, These mice may have applications in studies requiring conditional gene manipulation in central nervous system microglia, as well as other Cx3cr1-expressing myeloid cell populations,

 · Mutation details: A targeting vector was designed to replace exon 2 of the chemokine C-X3-C receptor 1 Cx3cr1 gene with a cre/ERT2 cre recombinase fused to an estrogen receptor ligand binding domain coding sequence followed by an internal ribosome entry site IRES and an enhanced yellow fluorescent protein EYFP A frt-flanked neomycin resistance cassette, in reverse orientation to

Cx3cr1 CreERT2-driven Atg7 deletion in adult mice induces

Cx3cr1-CreERT2

 · LE-TgCX3CR1-Cre-ERT23Ottc Primer Name: CX3CR1 F103289 Primer Sequence 5′ to 3′: TCAGGGTGGCCCATAACCAC Primer Name: CreERT2 R713 Primer Sequence 5′ to 3′: AGGTAGTTATTCGGATCATCAGCTACAC These oligos produce a 1017 bp amplicon spanning the 5’ end of the CX3CR1-Cre-ERT2 using with OneTaq polymerase with 68oC annealing, Primer Name: CX3CR1 F103289 …

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Protocol for genotyping LE-TgCX3CR1-Cre-ERT23Ottc

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将creERT2-WPRE-polyA插入到小鼠Cx3cr1基因起始密码子处。 验证数据, 暂无数据, 发表文献, 暂无数据, 相关品系, 你也可能感兴趣, Tamoxifen诱导Cre-ERT2小鼠 使用指南, Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2

LC3-Associated Endocytosis Facilitates β-Amyloid Clearance

Similarly immunofluorescence and flow cytometry analyses using a Cre-dependent reporter mouse line demonstrate activity of CreERT2 primarily in microglia upon tamoxifen administration with the caveat of activity in leptomeningeal cells Finally flow cytometric analyses reveal absence of EGFP expression and minimal activity of CreERT2 in blood monocytes of the

Targeting Microglia Using Cx3cr1-Cre Lines: Revisiting the

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cx3cr1 cre ert2

 · CX3CR1-cre ERT2 positive mice that have been injected with tamoxifen and have reconstituted the monocytic pool are referred to herein as MG-cre + mice MG-cre-animals used in the present study have been exposed to tamoxifen and allowed the same reconstitution period as the MG-cre + mice to ensure no off-target effects from tamoxifen administration, Mice used for bone marrow isolation and BMDM

Dorsal horn neurons release extracellular ATP in a VNUT

Mutation details: A cre/ERT2 cassette with a floxed neo gene was inserted by RedE/T recombineering into a BAC containing the Cx3cr1 locus replacing the coding exon of Cx3cr1 Properly targeted ES cells were aggregated with 8-cell embryos to generate chimeras, Chimeras were backcrossed to C57BL/6 mice,

Cx3cr1 Targeted Allele Detail MGI

LE-TgCX3CR1-Cre-ERT23Ottc transgenic rats January 08 2019 This protocol was updated on 01-08-2019 by CR Any questions regarding protocol contact nidatransgenicprojects@mail,nih,gov, Genomic DNA Preparation by Macherey-Nagel Tissue Spin Columns Using this kit according to the manufacturer’s protocol is the preferred way for preparing genomic DNA at OTTC when it is intended for ddPCR i,e

 · The Cx3cr1 cre and Cx3cr1 creER mice were crossed to R26-yfp mice in which irreversible induction of YFP expression is induced upon Cre recombinase expression and activation respectively B and C Flow cytometric analysis of CX 3 CR1 + B and CX 3 CR1 − C mononuclear phagocyte populations of Cx3cr1 gfp/+ Cx3cr1 cre/ + :R26-yfp and Cx3cr1 creER/+ :R26-yfp mice

Tmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for

These data suggest that in this Cx3cr1-CreERT2J mouse line Cre-ERT2 recombinase possesses some basal enzymatic activity independent of tamoxifen However the Cre-dependent expression is nearly completely restricted to microglia Fig 3A–F Download figure; Open in new tab; Download powerpoint ; Figure 3 Basal expression of cre recombinase activity and microglial specificity of Cx3cr1

Cx3cr1 Targeted Allele Detail MGI

A cre/ERT2 cassette with a floxed neo gene was inserted by RedE/T recombineering into a BAC containing the Cx3cr1 locus, replacing the coding exon of Cx3cr1, Properly targeted ES cells were aggregated with 8-cell embryos to generate chimeras, Chimeras were backcrossed to C57BL/6 mice, Tne neo was excised by crossing the animals to a cre deleter strain TgPgk1-cre1Lni,