ins 1 cells – ins 1 cell line
· Conversely INS-1 cells have been used in a recent study to explore suppressive effects of 17beta-estradiol via ERalpha ERbeta and G-protein coupled ER on lipogenesis obviously assuming presence of operational ERs, Whether or not lack of effect of BPA on GSIS in INS-1 832/13 cells is the consequence of differences in ER expression patterns remains to be determined,
Cell Culture Rat Insulinoma Ins 1 Cells
Ins1 Cells
INS-1 A and HeLa B cells were incubated without or with siRNA targeting HMGA1 100 pmol and transfected thereafter with 1 μg of mouse pm INSII-Luc or human ph INS-Luc Luc reporter plasmid, respectively, either in the absence or presence of effector vectors 0,5 μg each for HMGA1, PDX-1, and MafA, Values are expressed relative to the Luc activity obtained in transfections with the Luc reporter plasmid alone slashed bar that is assigned an arbitrary value of 1…
Ins 1 Cells, supplied by Millipore, used in various techniques, Bioz Stars score: 96/100, based on 1 PubMed citations, ZERO BIAS – scores, article reviews, protocol conditions and more, https://www,bioz,com/result/ins 1 cells/product/Millipore, Average 96 stars, based on 1 article reviews, Price from $9,99 to $1999,99,
Insulin-secreting cell lines: classification
ins 1 cells
ins-1 Cells, ins-1 Cells, Organism Type: Rat: Insulin secreting beta cell derived line, Tissues: Pancreas: Phenotype: Adherent: Primary: no: Application : Cell Culture / Growth Conditions,Stable Cell Transfection,Transient Transfection: Every Step of the Way, a Wide Range of Cell Health Products, Maintaining healthy cells is the key to experimental success and reproducible research results, To
Phenotype : Adherent
INS‑1 cells were stimulated with lipopolysaccharide Apoptosis and reactive oxygen species ROS formation were measured using flow cytometry and cell proliferation was measured using Cell Counting Kit‑8 solution Autophagy was assayed using western blotting and transmission electron microscopy, The expression levels of interleukin‑1β IL‑1β and caspase‑1 were detected by western blotting, The results demonstrated that …
The most widely used insulin-secreting cell lines are RIN HIT beta TC MIN6 and INS-1 cells These cells contain mainly insulin and small amounts of glucagon and somatostatin RIN cells except for the subclone RIN-38, are not glucose-responsive, HIT cells and beta TC cells secrete insulin in response to glucose, but their dose-response curve is markedly shifted to the left MIN6, INS-1 and a newly available subclone of beta …
Bisphenol A induces oxidative stress-associated DNA damage
Stimulation of EDG receptors in islets and INS-1 cells with SPP inhibited glucagon-like peptide 1 GLP-1-stimulated cAMP production and insulin secretion in a concentration-dependent manner Pertussis toxin antagonized the SPP effects on insulin release Thus EDG receptors are expressed in pancreatic islet beta-cells and Gi seems to mediate the inhibition by SPP of adenylyl cyclase and cAMP formation and inhibition …
Ins 1 Cells
Ins1 Cells, supplied by ATCC, used in various techniques, Bioz Stars score: 99/100, based on 1 PubMed citations, ZERO BIAS – scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 1 PubMed citations,
ins-1 Cells
INS-1
INS-1 cells were exposed to different doses of BPA 0 25 50 100 μM We conducted the single-cell gel electrophoresis SCGE assay to measure DNA damage and studied proteins such as p53 and p-Chk2 T68 by Western blotting in order to verify the genotoxicity of BPA, Moreover, we examined intracellular reactive oxygen species ROS and glutathione GSH to discuss the possible mechanism of DNA damage, The …
Insulin Release Ins 1 Cells
Ins‐1 cells were treated with LB at different concentrations 10 ‐5 , 10 ‐6 , 10 ‐7 , 10 ‐8 , 10 ‐9 mol/L for 4 h, B Effects of different treatment time of LB on insulin secretion, Ins‐1 cells were treated with LB 10 ‐6 mol/L for different time 0‐24 h, The levels of insulin were measured with immunoradiometric assay method, C Effects of different concentration of LB on the viability of Ins‐1 cells, Ins‐1 cells were treated with LB at different …
Liraglutide Improves the Survival of INS-1 Cells by
The B-domain, on the other hand, binds an islet cell-specific protein with characteristics similar to those of Isl-1, a transcriptional activator protein that binds to the E2 enhancer of the rat insulin-1 gene , INS1 insulin 1, which has been previously assigned to rat chromosome 1, was not linked to these markers ,
Evaluation of the INS-1 832/13 Cell Line as a Beta-Cell
FOXM1: A gene on chromosome 12p13 that encodes a member of the forkhead family of transcription factors, which is thought to play a role in controlling cell proliferation,
Endothelial differentiation gene receptors in pancreatic
· We incubated INS-1 cells with 05 mmol l–1 PA in the presence of 5 mmol l–1 glucose for 24h The viability of INS-1 cells was reduced dramatically with the treatment of PA by CCK8 assay As shown in Figure 1 the INS-1 cells viability was significantly decreased to 55% in PA group compared with the control group Figure 1 INS-1 cells were then treated and engorged with Liraglutide to determine if Liraglutide protected INS-1 cells from death The survival of INS-1 cell …
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Role of autophagy in LPS‑induced inflammation in INS‑1 cells
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